33 US AI Startups That Secured Massive $100M+ Funding Rounds in 2025 – The Full List Revealed!
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33 US AI Startups That Secured Massive $100M+ Funding Rounds in 2025 – The Full List Revealed!

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Summary:

  • In 2025, 33 US AI startups have raised $100 million or more in funding, showcasing massive investor interest.

  • Notable rounds include OpenAI's $40 billion raise at a $300 billion valuation and Thinking Machines Lab's $2 billion seed round.

  • Key investors like Andreessen Horowitz, Kleiner Perkins, and Lightspeed Venture Partners are heavily involved across multiple deals.

  • Sectors such as healthcare AI, legal tech, and AI infrastructure dominate the funding landscape with high valuations.

  • The trend indicates a continuation of 2024's momentum, with several companies raising multiple rounds in a single year.

The AI Funding Boom Continues in 2025

Last year was monumental for the AI industry in the U.S. and beyond, with 49 startups raising funding rounds worth $100 million or more in 2024. As we approach the end of the third quarter of 2025, the momentum shows no signs of slowing down. Multiple billion-dollar rounds have already been announced, and several companies have raised more than one mega-round this year.

Here's a comprehensive list of all the U.S. AI companies that have raised at least $100 million in 2025, organized by month:

August

  • EliseAI, a healthcare and housing automation platform, raised $250 million in a Series E round led by Andreessen Horowitz, valuing the startup at $2.2 billion.
  • Decart, an AI research lab, raised $100 million at a $3.1 billion valuation, with participation from Sequoia Capital, Benchmark, and Zeev Ventures.

July

  • Fal, a generative media platform, raised a $125 million Series C round led by Meritech Capital Partners, valuing the company at $1.5 billion.
  • Ambience Healthcare raised $243 million in a Series C round led by Oak HC/FT and Andreessen Horowitz for its AI healthcare operating system.
  • Reka AI secured $110 million in a Series B round with investors like Snowflake and Nvidia, reaching a $1 billion valuation.
  • Thinking Machines Lab confirmed a massive $2 billion seed round led by Andreessen Horowitz, valuing the company at $12 billion.
  • OpenEvidence raised $210 million at a $3.5 billion valuation for its AI-powered search tool for clinicians, led by Kleiner Perkins and GV.
  • Harmonic raised a $100 million Series B round led by Kleiner Perkins for its mathematical reasoning engine, valued at $875 million.

June

  • Abridge, a healthcare AI unicorn, announced a $300 million Series E round led by Andreessen Horowitz, doubling its valuation to $5.3 billion.
  • Harvey raised its second $300 million round of 2025, co-led by Kleiner Perkins and Coatue, bringing its valuation to $5 billion for AI legal tools.
  • Tennr raised $101 million in a Series C round led by IVP for healthcare referrals, valued at $605 million.
  • Glean landed a $150 million Series F round led by Wellington Management, valuing the enterprise search startup at $7.25 billion.
  • Anysphere raised a $900 million Series C round led by Thrive Capital for its AI coding tool Cursor, valued at nearly $10 billion.

May

  • Snorkel AI announced a $100 million Series D round led by Addition for AI data labeling, valued at $1.3 billion.
  • LMArena raised a $100 million seed round co-led by Andreessen Horowitz and UC Investments for AI benchmarking, valued at $600 million.
  • TensorWave raised $100 million in a Series A round co-led by Magnetar Capital and AMD Ventures for AI infrastructure.

April

  • SandboxAQ closed a $450 million Series E round with investors like Nvidia and Google, valued at $5.7 billion.
  • Runway raised $308 million in a Series D round led by General Atlantic for AI media models, valued at $3 billion.

March

  • OpenAI raised a record-breaking $40 billion funding round led by SoftBank, valuing the startup at $300 billion.
  • Nexthop AI raised $110 million in a Series A round led by Lightspeed Venture Partners for AI infrastructure.
  • Insilico Medicine raised $110 million in a Series E round for AI-driven drug discovery, valued at $1 billion.
  • Celestial AI raised $250 million in a Series C round led by Fidelity for photonic AI infrastructure, valued at $2.5 billion.
  • Lila Sciences raised a $200 million seed round led by Flagship Pioneering for a science superintelligence platform.
  • Reflection.Ai raised $130 million in a Series A round led by Lightspeed Venture Partners and CRV for autonomous systems, valued at $580 million.
  • Turing closed a $111 million Series E round led by Khazanah Nasional for AI coding, valued at $2.2 billion.
  • Shield AI raised $240 million in a Series F round co-led by L3Harris Technologies and Hanwha Aerospace for defense tech, valued at $5.3 billion.
  • Anthropic raised $3.5 billion in a Series E round led by Lightspeed for AI research, valued at $61.5 billion.

February

  • Together AI raised $305 million in a Series B round co-led by Prosperity7 and General Catalyst for open-source AI, valued at $3.3 billion.
  • Lambda raised $480 million in a Series D round co-led by SGW and Andra Capital for AI infrastructure, valued at nearly $2.5 billion.
  • Abridge raised $250 million in a Series D round co-led by IVP and Elad Gil for patient transcription, valued at $2.75 billion.
  • Eudia raised $105 million in a Series A round led by General Catalyst for AI legal tech.
  • EnCharge AI raised $100 million in a Series B round led by Tiger Global for AI hardware.
  • Harvey raised $300 million in a Series D round led by Sequoia for legal AI, valued at $3 billion.

January

  • ElevenLabs raised $180 million in a Series C round co-led by ICONIQ Growth and Andreessen Horowitz for synthetic voice, valued at over $3 billion.
  • Hippocratic AI raised $141 million in a Series B round led by Kleiner Perkins for healthcare LLMs, valued at over $1.6 billion.

This list highlights the continued investor confidence in AI startups, with significant funding across various sectors including healthcare, legal tech, infrastructure, and more.

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